Following the results of the 2025 J.LEAGUE YBC Levain CUP 3rd Round held today, kickoff times and venues for the Play-off Round have been decided as follows.
| Date | Kickoff | Fixture | Venue | |
| 1st Leg |
Wednesday, June 4 | 19:00 | Tokyo Verdy vs. Kashiwa Reysol | Ajinomoto |
| 1st Leg |
Wednesday, June 4 | 19:00 | Shonan Bellmare vs. Jubilo Iwata | LemonSHiratsuka |
| 1st Leg |
Wednesday, June 4 | 19:00 | Cerezo Osaka vs. Yokohama FC | YODOKO STADIUM |
| 1st Leg |
Wednesday, June 4 | 19:00 | Avispa Fukuoka vs. Sanfrecce Hiroshima | BEST-STA |
| 2nd Leg |
Sunday, June 8 | 14:00 | Jubilo Iwata vs. Shonan Bellmare | Yamaha |
| 2nd Leg |
Sunday, June 8 | 15:00 | Sanfrecce Hiroshima vs. Avispa Fukuoka | E. PEACE |
| 2nd Leg |
Sunday, June 8 | 18:00 | Yokohama FC vs. Cerezo Osaka | NHK SPRING .F |
| 2nd Leg |
Sunday, June 8 | 19:00 | Kashiwa Reysol vs. Tokyo Verdy | SANKYO FKashiwa |
●Date
1st Leg Wednesday, June 4
2nd Leg Sunday, June 8
●Competition Format
Seven winning teams for 3rd Round, and Sanfrecce Hiroshima participating in AFC Champions League Two 2024/25 will play home and away format in the Play-off Round.
●Match format and determination of winners
In case a 90-minute match (two halves of 45 minutes each) is played and no winner is decided, an extra 30 minutes (two halves of 15 minutes each) is played. If the winner is still not decided, the winner is decided by a penalty shoot-out.
